Metabolomics Platform

Metabolomics is the newest and most recent of the various omic sciences to have emerged. This branch of science is responsible for the systematic study of the metabolomic profile of a biological process. Metabolomics studies the metabolic profile in biological samples (fluids, tissues, cell cultures, etc.) for the purpose of discovering diseases and risk factors and determining biomarkers. This science is also useful in determining nutritional therapies and interventions.

• The Metabolomics Platform is shared equipment created by the Universitat Rovira i Virgili of Tarragona and CIBERDEM. It is also part of the Instituto de Investigación Sanitaria Pere Virgili (IISPV). It is located in the Scientific and Technical Resource Service building of Universitat Rovira i Virgili (Sescel·lades, Tarragona Campus ).

The purpose of the CIBERDEM Metabolomics Platform is to provide service to different research groups, such that metabolomics can be included as a complementary tool for their work.

www.metabolomicsplatform.com

Services

• Experiment Analysis: Assistance in designing experiments as regards the attainable objectives, suitable statistical sampling, number of samples to be processed, logistic aspects, etc., in order to obtain satisfactory results.
• Chemical Analysis: Measurement of metabolites in human and animal biofluids (serum, urine, cerebrospinal fluid, etc.), by means of high-resolution NMR. There are also probes available for measuring biological tissues, semisolid tissues, etc.
• Result Analysis: Statistical analyses of metabolites (metabolic fingerprint) are performed in population studies to classify and differentiate healthy populations from populations suffering diseases and to determine at-risk populations. The metabolomic profiling technique and the search for biomarkers (metabolic targeting) based on comparing analytical measurements with the information in databases are also addressed.

Infrastructure and Equipament

MASS SPECTROMETRY EQUIPMENT: 

• EXACTIVE GC HRMS
• ORBITRAP ID-X 
• MALDI-HRMS 
• LC-Q-TOF/MS (AGILENT IFUNNEL 6550) 
• 2 X LC-QQQ (AGILENT IFUNNEL G6490AA) 
• GC-QQQ (AGILENT G7010AA); GC- Q-TOF (AGILENT  G7200AA) 
• GCXGC-TOFMS (LECO PEGASUS 4D) 
• MALDI-TOF/TOF (BRUKER ULTRAFLEXTREME)

HR-NMR EQUIPMENT: 

• 600 MHZ BRUKER AVANCE III + CRYOPROBE + SCANJET 
• 500 MHZ BRUKER AVANCE III + HR-MAS PROBE